#ABS Plus) 4s3 ™ Semi-Automatic Sheet Heat Sealer (Brooks Life Sciences cat. Instrument settings can be saved as a protocol file. Data collection speed is sufficient for running fast cell-based kinetic assays. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. Instrument settings can be saved as a protocol file. This document is provided to customers who have purchased Molecular Devices, Inc. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices SpectraMax® microplate readers support a multitude of assays for nucleic acids and protein detection. Absorbance, fluorescence, and luminescence detection with upgradeable high-performance capabilities. 6") Weight. 2. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Weight. SpectraMax i3x. the standard plate reader applications, protecting your initial investment. The advantage of using labeled probes is detection specificity. 0200-6262. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 9000-0756. Important things to consider when purchasing a multimode microplate reader. is user upgradeable—from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on . Company Molecular Devices, LLC. To update the driver. What plates do you recommend for SpectraMax Plate Readers? Luminescence : Solid white plates for top reads and white plates with clear bottoms for. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Next generation SpectraMax® absorbance microplate readers. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices Supplier: Molecular Devices Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength. BMG Labtech - THERMOstar. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. PURPOSE. Find new and used lab equipment and shop a wide selection of products and vendors. US HEADQUARTERS. The FlexStation uses a Xenon flashlamp and monochromators for both excitation and emission light to allow the use of a wide range of. For SpectraMax ABS Plus reader, r 2 = 0. * Note: Programming the washer with a final aspirate height of 4. The ability to accurately quantitate cell number in multi-well microplates enables a multitude of biological applications that study cell health or proliferation. 1 sec. Endpoint, dual read, kinetic, fast kinetic. The instrument will read theThe FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Plus 384. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. absorbance plate readers. Tests every subsystem of the reader or . For more sample throughput, the system can be easily integrated into a full roboticOnly Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. This can increase throughput and efficiency, thus saving time and space in the lab. With The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 8×15×8. 9. Learn how you can utilize microplate readers to drive landmark discoveries. The chamber contains springs that position the cuvette in the proper alignment for a read. When connecting for the first time to either the SpectraMax L reader or StakMax system. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. What are the Vmax plate reader specifications? Recommended Articles SoftMax® Pro 7. 9000-0756. MycoAlert Substrate was then added to the wells, and the plate was incubated for 10 minutes at room temperature. SpectraMax M5. Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableSpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 635. 1. Supplier: Molecular Devices. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. VMax® Microplate Readers. 5 hours. Robust, high-value microplate readers that can run samples based on pre-defined. Instrument settings can be saved as a protocol file. First, as mentioned before, the walkaway time significantly. The adapter is required for optimum performa nce with standard 96-well and 384-. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. SpectraMax® Microplate Readers and SoftMax® Pro Software. One plate carrier adapter is provided with the instrument. Every step is optimized to simplify analysis and reporting to support our microplate readers. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. integration time per well, depending on firmware version. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Methods. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. For a higher . TheUnparalleled performance on a personalized platform. 25 to 3. 5 µL of each sample was pipetted into a 96-well. 304 signal level saturation. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 1. 1. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. Plate reader instructions Computer. Product Detail. 1 - 16 of 69. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The SpectraMax ABS Plus reader utilizes Molecular . STAKMAX. Choose the desired plate type, using the upper dropdownThe Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. Turn the reader on and secure the USB cable. 2. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. V. Get a front row view of your cells. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The plate was then re-read on the plate reader (Read B). Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 306 plate air cal. 0077 OD 0. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. FAST AND ACCURATE METERTECH Microplate Reader $3,650. The computer controlling the plate reader runs Windows XP. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. ) Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Molecular Devices’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. 1. Clone Screening. The plate reader optics are tunable for absorbance,. Maintaining data integrity is a. Figure 1. 6 kg (30 lbs. to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and. Read height should be optimized for Vmax® plate reader specifications. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Fluorescence Polarization (FP) Glow Luminescence (Lumi) The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast,. or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. 50-plate magazine : 0200-6187. Find our technical support information along with contact details. Instrument settings can be saved as a protocol file. Ratios for each sample were calculated as Read B/Read A. 9000-0756. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. The SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Figure 9. A). Wait 20 seconds. 2. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 2. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Additional high-quality filters to supplement your assay wavelength range requirements are available directly from Molecular Devices. the iD5 reader’s filter system. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. 0. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Robust, high value microplate reader designed to deliver research-grade results to any laboratory. 1 sec. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Integrated to. Published Dec 16, 2020 | Updated Dec 16, 2020. Longside barcode reader: 0310-5101. PURPOSE. Endpoint. 1. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. EMax. EUROPE. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesMolecular Devices Software. Ensure data accuracy of your reader. . Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. SoftMax® Pro 7. Shortside barcode reader: 0310-5102. Sale price $3,495. The system provides automation for up to 50 microplates for easy walkaway automation. Here, we describe a novel GCaMP6s-CAAX-based calcium assay utilizing a high-throughput fluorometric imaging plate reader (Molecular Devices, Sunnyvale, CA) format that can identify both activators and inhibitors of T-type Ca 2+ channels. The former have been the traditional domain of plate readers and great strides have been. Microplate Stacker and Washer. Supersedes 1. Cellular Imaging Systems. Molecular Devices Software. Under "Category", select "Plate Type". The plate is set up for reading and images are acquired according to specified parameters. Molecular Devices SpectraMax Gemini XS Microplate Reader. Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on. Restart the system. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. These systems are hybrids that allow you to choose between. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). Flip open the carriage door and using a flash light to check where the plate is stuck and whether it has been damaged. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. For customers outside the U. SpectraDrop High-Throughput Screening (HTS) Kit. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader (Molecular Devices, USA) at each timepoint. Extended linear dynamic range. Plate chamber design of the SpectraMax category 1 microplate readers. SpectraTest® Validation Package User Manual Download Page. AXON Patch-Clamp. Please Inquire. 1. 2. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Select Start>Control panel>Device Manager. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. (7) Anastellin impacts on the processing of extracellular matrix fibronectin and stimulates release of cytokines from coronary artery smooth muscle cells. Category 2 and 3 microplate readers use gradient heating as well. S. ” So, acquisition and analysis come from one software system. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Explore our fully-integrated,. Supplier: Molecular Devices. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Since cells are nearly confluent, the protocol. Supersedes 1. For nearly 30 years, Molecular Devices has provided scientists with . Flexibility to read a variety of plate formats. absorbance plate readers. 40266 Email: drugdiscoverytech@lifetech. Plate Formats 6 to 1536 wells. Select Update Driver. together with a Molecular Devices plate. Shop a wide selection of Molecular Devices FLIPR TETRA System's on LabX. Pre-defined protocols. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. Retrieving Each. and the assembly was incubated for 5 minutes in the dark prior to reading the plate. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 7°C and the bottom to 36. Summary of Contents for Molecular Devices SpectraMax M2. If the computer is connected to the Internet: Select Search Automatically for. The plate was then read on Molecular Devices microplate readers with luminescence detection mode (Read A). Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofThe plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and mixed. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. , attenuation mode = out. S. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. Clone Screening. S. Page 1 of 8 . Molecular Devices SoftMax ® Pro software was used to analyze and present the data. 1700 +1 800. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. 5. SpectraMax Microplate Readers. Read one sample or up to 384 in a. 72 MB. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Fluorescence Microplate Reader Comparison Chart. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Page 1 of 8 . plate type, read area and number of images per well, plus specify positive/negative wells. Temperature Control 4°C above ambient to 45°C. 5 sec. 1) is a fluorescence plate reader that can measure intracellular Ca 2+ concentration using both single- and dual-wavelength fluorescent probes. Users of Molecular Devices' SpectraMax® i3 Multi-mode reader can even add an optional. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. Three low volume 24-well bottom slides. Title: F3/F5 Multi Mode Microplate Readers | Molecular Devices Author: Molecular Devices, LLC Subject: Learn how the F3/F5 Multi-mode microplate readers can utilize its filter sets to accurately perform measurements with up to five modes of detection. Molecular Devices SpectraMax Gemini XS microplate reader with laptop and software (Pre-owned) $5,500. FLIPR System with the 540-590 nm BP Emission FLIPR Filter installed (Molecular Devices Corporation) Membrane Potential Assay Kit (Molecular Devices Corporation, R-8034) Clear, flat-bottom, black- or clear-wall 384- or 96-well plates (see consumables list in Appendix A) Clear, polypropylene compound plates (see consumables list in Appendix A) SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. SoftMax Pro Software. This process should take about 3 - 5 minutes. PathCheck sensor corrects optical pathlength & pipetting variation. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Learn more about their features and specifications in. We would like to show you a description here but the site won’t allow us. Molecular Devices' microplate readers. FlexIII. Chapter 1: Introduction The SpectraMax® iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Supplier: Molecular Devices. Compact designs. Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. SoftMax® Pro 7. Changing Plate Hold-Down Devices 123 Cell Reservoir 125 Chapter 7: Calibration and Signal Test 127. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 313 reference gain check fail. The instrument settings were identical to those used in Example 1 above. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. Fill a clean plate with water. VMax . Automated microplate readers reduce manual manipulation and ensure consistent operation. 2. Imaging System Giles Scientific Biomic V3 Automatic Plate. +1 800-635-5577. Shop now. Please click on the links below to view the Microplate Reader Comparison Tables. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. SpectraMax MiniMax 300 imaging cytometer enables quick imaging and analysis of cells and helps in observing the viability of your cells with the field-upgradeable cellular imaging for spectramax i3/i3x multi-mode detection platform. Cells in each image are identified by SoftMax Pro Software and cell-by-cellOne plate carrier adapter is provided with the instrument. ~ Bio-Tek ELx808 Microplate Reader $2,795. Versa. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. The instruments feature a bottom-read mode ideal for whole-cell-based assays, and may beSoftMax Pro GxP Software. Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm) with 4 filter choices included at time of purchase. In an analogous manner, the same sample names were assigned to a CuvetteSet section. Versa Max. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The system features 96, 384, and 1536 simultaneous liquid transfer, multi-wavelength kinetic reading, fluorescence and luminescence detection, agile internal plate handling, and elevated instrument0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. Search. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. edu. 190. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. Absorbance Plate Reader Comparison Chart. Test up to 3. READ ANALYZE IMPORTa single plate read using any standard cuvette, or 96- or 384-well microplate. 2 GxP Software is our most secure software to help you achieve full FDA 21 CFR Part 11 compliance with streamlined workflows to ensure data integrity. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The Synergy H4 is a convenient benchtop microplate reader that offers robust. 1. Endpoint, kinetic, spectrum. to effectively test for endotoxins in samples. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. #0200-7011) Instrument setupSOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. This document is provided to customers who have purchased Molecular Devices, Inc. 0A Sunnyvale, CA Made in USA Microplate readers read biological, chemical or physical events that occur on a microtiter plate. Plate reader. A control well was set up to verify the accuracy of the standard curve. 0%, raw data units = counts/sec. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Services. The drawing shows a standard microplate of 15 mm height covered with a lid. 0. Supplier: Molecular Devices. Plate stacker and robot integration The SpectraMax 340PC384 reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. with the kit reagent and transferred to the plate reader for evaluation at the same time. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. A standard spectrophotometer measures the absorbance of one sample at a time. Cells were thawed and propagated according to the recommended method specified by the supplier. Also see: SoftMax® Pro Microplate Software User Manual Download Page. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is preferred for luminescence rather than the core’s multimode readers. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Imaging with the MiniMax 300 Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The opaque walls prevent stray light from being detected from neighbouring. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. . 9. This multi plate reader is excellent to work with for checking the U. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes wi. Supersedes 1. +44-118-944-8000. An ampicillin-resistant E.